Potential Role of WIP Family Genes in Drought Stress Response in Rubus idaeus

Xiangqian Gao, Guiyan Yang, Dapei Li, Muhong Xie, Yujie Mei, Lan Hu, Yongqi Zheng () and Evangelia V. Avramidou ()
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Xiangqian Gao: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Guiyan Yang: Laboratory of Walnut Research Center, College of Forestry, Northwest A&F University, Yangling 712100, China
Dapei Li: Laboratory of Walnut Research Center, College of Forestry, Northwest A&F University, Yangling 712100, China
Muhong Xie: Laboratory of Walnut Research Center, College of Forestry, Northwest A&F University, Yangling 712100, China
Yujie Mei: College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
Lan Hu: College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
Yongqi Zheng: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Evangelia V. Avramidou: Laboratory of Forest Genetics and Biotechnology, Institute of Mediterranean Forest Ecosystems, ELGO-DEMETER, 11528 Athens, Greece

Agriculture, 2024, vol. 14, issue 11, 1-13

Abstract: Rubus idaeus is one of the primary cultivated species of raspberries, renowned for its appealing color, distinctive flavor and numerous health benefits. WIP proteins, which contain three conserved amino acids (W: Tryptophan, I: Isoleucine, P: Proline) and four zinc finger motifs in a highly conserved C-terminal region, are members of the A1d subgroup of C2H2 zinc finger proteins. Drought is one of the main limiting factors of plant growth and development, which restricts the cultivation and utilization of raspberry in northwest China. In this study, to obtain candidate genes for drought resistance, we identified key related genes, RiWIPs , from R. idaeus and analyzed their bioinformation and tissue stress response expression to drought. We found that there are three RiWIPs in R. idaeus and they are located on chromosomes 3, 4 and 6 of R. idaeus , respectively. The open reading frames (ORFs) of the RiWIPs ranged from 870 to 1056 bp in length, encoding 289 to 372 amino acid residues. The proteins were highly conserved and feature diverse conserved motifs. The promoters of the RiWIPs contained abundant cis -elements related to growth, development and stress response. Tissue-specific expression analysis revealed that the RiWIPs were expressed in the leaves, stems and roots of both drought-susceptible and drought-tolerant cultivars, except for RiWIP2 , which was only expressed in the roots of the drought-tolerant one. Under drought stress, the transcriptional activity of the RiWIPs was increased to different degrees with specificity in the leaves, stems and roots. Our study demonstrated the role of WIP genes in raspberry drought response and provided a marker gene, RiWIP2 , for drought resistance and candidate genes for subsequent drought-resistant breeding of R. idaeus .

Keywords: Rubus idaeus; WIPs; bioinformatics; drought stress; transcriptional activity (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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