Transcriptomic and Metabolomic Insight into the Roles of α-Lipoic Acid in the Antioxidant Mechanisms of Sheep

Zhaohui Yao, Gaiying Li, Xinlu Yang, Liyang Zhang, Chuanyou Su, Tong Fu, Linfeng Wang, Hongxia Lian, Tengyun Gao and Kaizhen Liu ()
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Zhaohui Yao: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Gaiying Li: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Xinlu Yang: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Liyang Zhang: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Chuanyou Su: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Tong Fu: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Linfeng Wang: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Hongxia Lian: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Tengyun Gao: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Kaizhen Liu: Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China

Agriculture, 2024, vol. 14, issue 11, 1-9

Abstract: Alpha lipoic acid (α-LA) is a powerful antioxidant, which can reduce oxidative damage and inflammation in the host. In previous research, we found that 600 mg/kg α-LA supplemented in the diet could improve the activity of antioxidant enzymes and regulate the secretion of inflammatory factors in rumen of sheep. However, the mechanism of α-LA driving the antioxidant capacity in rumen of sheep remains unknown. The aim of this current research was to investigate the role of α-LA in antioxidant and inflammatory processes in the rumen of sheep. Transcriptomic and metabolomic analyses were performed to assess the variation of genes and metabolites of rumen epithelial tissue in sheep induced in the LA (600 mg/kg α-LA) group compared with the CTL (0 mg/kg α-LA) group. The results showed that some discovered core genes in the rumen epithelial tissue were negatively correlated with antioxidant activity. G6PD and HK2 , the downregulated and upregulated core genes in the comparison of LA/CTL, were enriched in the pentose phosphate pathway (PPP) and the pathways of mannose and oligosaccharide metabolism, respectively. The PPP is a metabolic pathway within cells, primarily functioning to produce nicotinamide adenine dinucleotide phosphate (NADPH) and ribose-5-phosphate. The differential metabolites were enriched in the pathway of 2-oxocarboxylic acid metabolism, which improves the antioxidant capacity of the rumen epithelium by increasing enzymatic activities of SOD. In conclusion, the present study demonstrated that α-LA improved antioxidant activity by regulating PPP and 2-oxocarboxylic acid metabolism. This study will provide a theoretical basis for the application of α-LA in the raising of sheep.

Keywords: α-lipoic acid; rumen epithelial tissue; transcriptomics; metabolomics; antioxidant (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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