Genetic Transformation of Common Beans ( Phaseolus vulgaris ): Achievements and Challenges

Matheus da Costa Moura, Patricia Valle Pinheiro (), Rosana Pereira Vianello, Natália Lima de Sousa, Josias Correa de Faria and Francisco José Lima Aragão ()
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Matheus da Costa Moura: Department of Molecular Biology, University of Brasilia, Brasilia 70910-900, Brazil
Patricia Valle Pinheiro: Embrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, Brazil
Rosana Pereira Vianello: Embrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, Brazil
Natália Lima de Sousa: Embrapa Recursos Genéticos e Biotecnologia, Brasilia 70770-917, Brazil
Josias Correa de Faria: Embrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, Brazil
Francisco José Lima Aragão: Embrapa Recursos Genéticos e Biotecnologia, Brasilia 70770-917, Brazil

Agriculture, 2024, vol. 14, issue 11, 1-18

Abstract: Genetic transformation is a valuable tool for the development of plant varieties with desirable traits that are present in the species germplasm with low genetic variability, i.e., resistance to pests and diseases and nutritional improvements. Although transgenic and edited crops have been successfully obtained for many plant species, it remains difficult for common beans ( Phaseolus vulgaris ), due to their recalcitrance to in vitro regeneration. This review discusses various methods employed, such as Agrobacterium -mediated transformation, biolistic (particle bombardment), and hairy root systems, noting their respective efficiencies and limitations. While there has been progress, including the development of the first transgenic common bean cultivar approved for commercialization (Embrapa 5.1), the article emphasizes the need for improved protocols and techniques for more efficient genetic transformation. It also touches upon the potential of gene editing technologies like CRISPR/Cas9 in overcoming existing challenges and facilitating the development of resilient bean varieties.

Keywords: plant breeding; genetically modified organism; transgenic; in vitro regeneration; Leguminosae; Agrobacterium tumefaciens; gene editing; biolistic; hairy root; recalcitrant species (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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