Identification of Two R2R3-MYB Genes Involved in Flavan-3-Ols Biosynthesis as Modulated by Salicylic Acid Through RNA-Seq in Grape Berries ( Vitis spp.)

Fengchan Yu, Pengfei Zhai, Wenjing Ge, Runtian Zhou, Shuwen Zan, Jinjun Liang, Pengfei Wen () and Yu Gary Gao ()
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Fengchan Yu: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Pengfei Zhai: Department of Mechanical Engineering, Shanxi Institute of Technology, Yangquan 045000, China
Wenjing Ge: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Runtian Zhou: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Shuwen Zan: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Jinjun Liang: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Pengfei Wen: College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
Yu Gary Gao: South Centers, College of Food, Agricultural, and Environmental Sciences, The Ohio State University, 1864 Shyville Road, Piketon, OH 45661, USA

Agriculture, 2024, vol. 14, issue 11, 1-15

Abstract: Flavan-3-ols are plant secondary metabolites that play important roles in stress resistance. Our previous studies revealed that salicylic acid (SA) activates R2R3-MYB transcription factors, promoting flavan-3-ol biosynthesis. This study identified two R2R3-MYB genes that exhibited positive responses to both exogenous SA and were probably involved in flavan-3-ol biosynthesis through RNA-sequencing, functional enrichment analysis, and qRT-PCR. The results indicated that the contents of total flavan-3-ols and their monomers, (+)-catechin and (−)-epicatechin, in grape berries after exogenous SA application were substantially increased compared to those in the control. A total of 683 differentially expressed genes in response to exogenous SA treatment were identified using RNA-seq. KEGG analysis revealed enrichment of the ‘flavonoid biosynthesis’ and ‘plant hormone signal transduction’ pathways. A specific module highly associated with flavan-3-ol biosynthesis was identified by constructing a co-expression network. Two candidate genes ( VvMYB108B and VvMYB145 ) likely participating in flavan-3-ol biosynthesis were selected using qRT-PCR. Therefore, these two potential genes that respond to SA and putatively participate in flavan-3-ol biosynthesis were identified for the first time. These results lay a solid basis for a more profound understanding of the molecular regulation of flavan-3-ol biosynthesis in grapes.

Keywords: Vitis vinifera; salicylic acid; flavan-3-ols; RNA-seq; WGCNA (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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