Characterization and Expression Patterns of Heat Shock Protein 70 Genes from Paracoccus marginatus in Response to Temperature and Insecticide Stress

Yanting Chen, Jianwei Zhao, Mengzhu Shi, Fei Ruan, Jianwei Fu, Wanxue Liu () and Jianyu Li ()
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Yanting Chen: Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Jianwei Zhao: Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Mengzhu Shi: Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350002, China
Fei Ruan: Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Jianwei Fu: Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350002, China
Wanxue Liu: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Jianyu Li: Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

Agriculture, 2024, vol. 14, issue 12, 1-13

Abstract: The objective of this study was to identify the Hsp70s in Paracoccus marginatus and explore their roles in P. marginatus ’s resistance to temperature and insecticide stress. The full-length cDNA sequences of PmHsp70s were obtained by PCR cloning and sequencing. The physicochemical and structural characteristics of PmHsp70s were analyzed, and a phylogenetic tree was constructed. The gene expressions of PmHsp70s were detected using qRT-PCR to explore the impacts of temperature and insecticide stress on P. marginatus . A total of 12 PmHsp70s were identified and cloned. The amino acids encoded by PmHsp70s were found to contain highly conserved regions characteristic of the Hsp70 family. The subcellular localization results showed that the majority of PmHsp70s were located in the cytoplasm. A total of 13 unique conserved motifs were identified for the PmHsp70s, of which 9 were shared motifs. The phylogenetic tree showed that the 12 PmHsp70s could be clustered into five branches, with the closest evolutionary relationship observed with the Phenacoccus solenopsis . The expression of the majority of PmHsp70s was up-regulated in P. marginatus when subjected to heat stress, with the higher expression fold change observed for PmHsp70-9 , PmHsp70-11 , and PmHsp70-12 . The expression of specific PmHsp70s was notably suppressed under cold stress, whereas the expression of others was markedly enhanced. Upon exposure to chlorfenapyr and lambda-cyhalothrin, the expressions of PmHsp70-11 and PmHsp70-12 were significantly up-regulated with the highest expression fold change, respectively. The results revealed the significance of specific PmHsp70s in the resistance of P. marginatus to temperature and insecticide stress. This study improved our understanding of the mechanisms underlying P. marginatus ’s adaptive responses to unfavorable environmental conditions.

Keywords: papaya mealybug; Hsp70 family; expression profiles; thermal stress; insecticide exposure (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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