Differences in Gene Expression in Germinated Soybean Seeds Among Four Cultivars, as Identified by Single Seed High-Throughput RNA Sequencing

Kosuke Nakamura (), Takahiro Gondo, Shinji Chiba, Satoshi Akimoto, Jumpei Narushima, Kazunari Kondo, Yohei Sugano, Hidenori Tanaka, Masatsugu Hashiguchi, Yuh Shiwa and Ryo Akashi
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Kosuke Nakamura: Division of Foods, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan
Takahiro Gondo: Frontier Science Research Center, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan
Shinji Chiba: Division of Foods, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan
Satoshi Akimoto: Division of Foods, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan
Jumpei Narushima: Division of Biochemistry, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan
Kazunari Kondo: Division of Biochemistry, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan
Yohei Sugano: Department of Food Hygiene, Hokkaido Institute of Public Health, Kita 19-jo Nishi 12-chome Kita-ku, Sapporo 060-0819, Japan
Hidenori Tanaka: Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan
Masatsugu Hashiguchi: Faculty of Regional Innovation, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan
Yuh Shiwa: NODAI Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
Ryo Akashi: University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan

Agriculture, 2024, vol. 14, issue 12, 1-11

Abstract: Gene expression in individual germinated seeds of four different soybean ( Glycine max [L.] Merr.) cultivars, GL3494, OAC Kent, Williams 82, and Jackson, was examined using single seed high-throughput RNA sequencing (ssRNA-seq). The gene expression was similar between two individual seeds of the same cultivar, but different among individual seeds of the four cultivars. Notably, ssRNA-seq identified five genes that were not stably expressed in Williams 82, having either no detectable sequence reads or less than five sequence reads mapped to the exon regions of chromosomes in Williams 82. These findings were validated by reverse transcription polymerase chain reaction analysis. The study’s results demonstrate that gene expression in germinated seeds is unique to an individual seed from each soybean cultivar. This uniqueness may affect composition and content of nutrients in germinated soybeans used as food ingredients.

Keywords: germinated soybean; single seed; cultivar; ssRNA-seq; gene expression (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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