Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

B. Megala Devi, Samyuktha Guruprasath, Pooraniammal Balu, Anirudha Chattopadhyay, Siva Sudha Thilagar, Kanaga Vijayan Dhanabalan, Manoj Choudhary, Swarnalatha Moparthi and A. Abdul Kader Jailani ()
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B. Megala Devi: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Samyuktha Guruprasath: Department of Chemical and Biotechnology, Sastra Deemed University, Thanjavur 613401, Tamil Nadu, India
Pooraniammal Balu: Department of Chemical and Biotechnology, Sastra Deemed University, Thanjavur 613401, Tamil Nadu, India
Anirudha Chattopadhyay: Pulses Research Station, S.D. Agricultural University, Sardarkrushinagar 385506, Gujarat, India
Siva Sudha Thilagar: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Kanaga Vijayan Dhanabalan: Department of Biological Science, Purdue University, West Lafayette, IN 47907, USA
Manoj Choudhary: Plant Pathology Department, University of Florida, Gainesville, FL 32611, USA
Swarnalatha Moparthi: Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27606, USA
A. Abdul Kader Jailani: Plant Pathology Department, University of Florida, Gainesville, FL 32611, USA

Agriculture, 2024, vol. 14, issue 2, 1-31

Abstract: Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.

Keywords: RNA interference; double-stranded RNA; plant disease management; pest control; precision agriculture (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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