Toxicity of Ethyl Formate to Tribolium castaneum (Herbst) Exhibiting Different Levels of Phosphine Resistance and Its Influence on Metabolite Profiles

Changyao Shan, Xinyue You, Li Li, Xin Du, Yonglin Ren () and Tao Liu ()
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Changyao Shan: Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China
Xinyue You: Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China
Li Li: Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China
Xin Du: Harry Butler Institute, Murdoch University, Murdoch, WA 6150, Australia
Yonglin Ren: Harry Butler Institute, Murdoch University, Murdoch, WA 6150, Australia
Tao Liu: Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China

Agriculture, 2024, vol. 14, issue 2, 1-18

Abstract: Ethyl formate (EF), a naturally occurring fumigant, has attracted widespread attention owing to its low toxicity in mammals. Here, Direct Immersion Solid-Phase Microextraction (DI-SPME) was employed for sample preparation in mass spectrometry-based untargeted metabolomics to evaluate the effects on Tribolium castaneum (Herbst) strains with different levels of PH 3 resistance (sensitive, TC-S; moderately resistant, TC-M; strongly resistant, TC-SR) when exposed to a sub-lethal concentration (LC 30 ) of EF. The bioassay indicated that T. castaneum strains with varying PH 3 resistance levels did not confer cross-resistance to EF. A metabolomic analysis revealed that exposure to sublethal doses of EF significantly altered 23 metabolites in T. castaneum , including 2 that are unique to the species which remained unaffected by external conditions, while 11 compounds showed a strong response. A pathway topology analysis indicated that EF caused changes to several metabolic pathways, mainly involving fatty acids and their related metabolic pathways. This study showed that EF can induce highly similar metabolic responses in insects across varying levels of PH 3 resistance, suggesting that the mechanisms driving the toxicity of EF and PH 3 are distinct. These insights significantly extend our knowledge of the toxic mechanisms of EF and provide direct evidence for the efficacy of EF treatment for managing PH 3 resistance in insects.

Keywords: Tribolium castaneum (Herbst); metabolites; PH 3 resistance; ethyl formate; DI-SPME-GC/MS (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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