The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat ( Triticum aestivum L.) Seeds

Anita Bošnjak Mihovilović (), Snježana Kereša, Boris Lazarević, Snježana Topolovec Pintarić, Katarina Martinko, Zvjezdana Marković, Katarina Turkalj and Ivanka Habuš Jerčić
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Anita Bošnjak Mihovilović: Department of Plant Breeding, Genetics and Biometrics, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Snježana Kereša: Department of Plant Breeding, Genetics and Biometrics, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Boris Lazarević: Department of Plant Nutrition, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Snježana Topolovec Pintarić: Department of Plant Pathology, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Katarina Martinko: Department of Plant Pathology, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Zvjezdana Marković: Department of Viticulture and Enology, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Katarina Turkalj: Department of Plant Breeding, Genetics and Biometrics, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia
Ivanka Habuš Jerčić: Department of Plant Breeding, Genetics and Biometrics, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, 10 000 Zagreb, Croatia

Agriculture, 2024, vol. 14, issue 4, 1-12

Abstract: In vitro plants that are free of pathogens are crucial for biotechnological breeding methods. The present study investigates the effects of sterilization with sodium hypochlorite (NaClO) and the addition of Plant Preservative Mixture (PPM TM ) to the growth medium on pathogen elimination, germination, and seedling development of the winter wheat ( Triticum aestivum L.) variety Mara. The sterilization treatments differed in the duration of seed sterilization in 4% NaClO and the PPM concentration added to the growth medium. Pathogenic fungi of the genera Aspergillus , Cladosporium , Penicillium , and Bipolaris were completely eliminated when the seeds were sterilized in NaClO and placed on growth media with the addition of PPM. Extending the duration of the sterilization treatment with NaClO to 50 min reduced Fusarium contamination, while the interaction between the 50 min sterilization treatment with NaClO and the addition of PPM to the growth medium reduced Alternaria contamination. Our results suggest that PPM could complement sterilization procedures with NaClO in the introduction of highly infected wheat seeds in vitro. Seed germination was not affected by sterilization with NaClO or by the addition of PPM. However, PPM at a concentration of 4 mL L −1 had a negative effect on seedling development.

Keywords: fungal pathogens; in vitro culture; PPM; sterilization; wheat seed; Alternaria sp.; Aspergillus sp.; Cladosporium sp.; Penicillium sp.; Fusarium sp.; Bipolaris sp. (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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