Resistant S. aureus Isolates Capable of Producing Biofilm from the Milk of Dairy Cows with Subclinical Mastitis in Slovakia

Ján Király, Vanda Hajdučková, Gabriela Gregová (), Tatiana Szabóová and Emil Pilipčinec
Additional contact information
Ján Király: Department of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Vanda Hajdučková: Department of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Gabriela Gregová: Department of Public Veterinary Medicine and Animal Welfare, the University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Tatiana Szabóová: Department of Public Veterinary Medicine and Animal Welfare, the University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Emil Pilipčinec: Department of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia

Agriculture, 2024, vol. 14, issue 4, 1-16

Abstract: Staphylococcus spp. is the most common cause of mastitis, with a significantly low cure rate. Bacterial characteristics like adhesion and biofilm formation, as well as extracellular factors, can affect the pathogenesis of staphylococcal mastitis. The study’s objectives were to confirm S. aureus , assess their antibiotic resistance, identify methicillin resistance genes, verify biofilm formation, and detect biofilm-associated genes from bovine mastitis samples using multiplex PCR (mPCR). From 215 milk samples, six were confirmed as S. aureus . Most isolates were sensitive to all measured antibiotics. One isolate was identified as an inducible form of MLSB resistance (macrolides, lincosamides, and streptogramin B resistance), while the other two isolates were resistant to penicillins and carboxypenicillins. In S. aureus cultures used for methicillin resistance genotypic analysis by PCR, the mecA and mecC genes were not found. Biofilm formation phenotypes were determined in four strains. An mPCR analysis revealed that all strains of S. aureus carried icaABCD , agrA , srtA , fnbA , clfA , and clfB genes. Only in one isolate was the fnbB gene detected; the bap gene was not detected in any of the isolates. This emphasizes the importance of using appropriate treatment and continuous monitoring of S. aureus to prevent the spread of antibiotic-resistant strains in dairy cow farms.

Keywords: subclinical mastitis; Staphylococcus aureus; biofilm; multiplex PCR; MIC (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/14/4/571/pdf (application/pdf)
https://www.mdpi.com/2077-0472/14/4/571/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:14:y:2024:i:4:p:571-:d:1369440

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().