A Comparative Analysis of Microbial Communities in the Rhizosphere Soil and Plant Roots of Healthy and Diseased Yuanyang Nanqi ( Panax vietnamensis ) with Root Rot

Changyuan Chen, Yifan Cheng, Fangli Zhang, Saiying Yu, Xiuming Cui () and Yuanshuang Wu ()
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Changyuan Chen: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Yifan Cheng: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Fangli Zhang: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Saiying Yu: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Xiuming Cui: Yunnan Key Laboratory of Sustainable Utilization of Panax Notoginseng, Kunming 650500, China
Yuanshuang Wu: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

Agriculture, 2024, vol. 14, issue 5, 1-18

Abstract: Microbial communities are not only an important indicator of soil status but also a determinant of plant nutrition and health levels. Loss of microbial community ecosystem control can directly lead to microbial disease occurrence. During the process of Yuanyang Nanqi wild imitation planting, root rot diseases frequently occur, seriously affecting their yield and quality. Via amplicon sequencing, this study mainly compared the microbial community composition between the rhizosphere soil and roots of healthy and diseased Yuanyang Nanqi with root rot. The α-diversity showed that the microbial community diversity and abundance in the roots of diseased Yuanyang Nanqi were much lower than those of those in healthy specimens, while no significant difference was found in the rhizosphere soil. The β-diversity showed that the bacterial community in the Gejiu region and the fungal community in the Honghe region were significantly different from those in other regions. The species relative abundance map showed that there was no obvious difference in microbial community composition between the rhizosphere soil and roots of healthy and diseased Nanqi, but in diseased specimens with root rot, the proportions of Pseudomonas and Fusarium increased. Based on a functional prediction analysis of FUNGuild, the results showed that the Nanqi roots were mainly pathological saprophytic type and that their rhizosphere soil was mainly saprophytic type. The microorganisms in the roots of Yuanyang Nanqi tubers with root rot were also isolated and identified through the use of the culture method. The possible pathogenic strains were tested via anti-inoculation, and Fusarium oxysporum was identified as one of the main pathogenic fungi of Nanqi root rot, which was consistent with the amplicon sequencing results. These results will help us understand the change trend of microbial communities in healthy and diseased plants and analyze the pathogens involved, the pathogenesis, and the beneficial microorganisms, which would provide a theoretical basis for effective biological control.

Keywords: Yuanyang Nanqi; root rot disease; amplicon sequencing; microbial diversity; pathogenic fungi (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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