Nitrogen Fertiliser Effects on Grain Anthocyanin and γ-Oryzanol Biosynthesis in Black Rice

Manisha Thapa (), Lei Liu, Bronwyn J. Barkla, Tobias Kretzschmar, Suzy Y. Rogiers and Terry J. Rose
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Manisha Thapa: Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia
Lei Liu: Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia
Bronwyn J. Barkla: Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia
Tobias Kretzschmar: Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia
Suzy Y. Rogiers: NSW Department of Primary Industries, Wollongbar, NSW 2477, Australia
Terry J. Rose: Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia

Agriculture, 2024, vol. 14, issue 6, 1-11

Abstract: Accumulation of phytochemicals in vegetative tissue under nitrogen (N) stress as an adaptive strategy has been investigated in various crops, but the effect of applied N on grain phytochemicals is poorly understood. This study investigated the effect of applied N on the biosynthesis and accumulation of rice ( Oryza sativa L.) grain anthocyanin and γ-oryzanol under different ultraviolet-B (UV-B) conditions in a controlled pot trial using two distinct black rice genotypes. The response of grain anthocyanin and γ-oryzanol content to applied N was genotype-dependent but was not altered by UV-B conditions. Applied N increased grain anthocyanin and decreased γ-oryzanol content in genotype SCU212 but had no significant effect in genotype SCU254. The expression of the OsKala3 regulatory gene was significantly upregulated in response to applied N in SCU212, while the expressions of OsKala4 and OsTTG1 were unchanged. The expression of all three regulatory genes was not significantly affected in SCU254 with applied N. Key anthocyanin biosynthesis genes were upregulated in grain by N application, which indicates that the common increase in anthocyanin in vegetative tissues under N deprivation does not hold true for reproductive tissues. Hence, any future approach to target higher content of these key phytochemicals in grains should be genotype-focused.

Keywords: anthocyanins; γ-oryzanol; nitrogen; gene expression; MBW complex; black rice (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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