MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

Huafei Zhou, Min Chen, Baoyan Li, Haining Chen, Hongtao Wang, Shaoli Wang, Binghui Luan and Baoyou Liu ()
Additional contact information
Huafei Zhou: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Min Chen: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Baoyan Li: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Haining Chen: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Hongtao Wang: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Shaoli Wang: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Binghui Luan: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China
Baoyou Liu: Institute of Plant Protection and Resource Environment, Yantai Academy of Agricultural Sciences, Yantai 265500, China

Agriculture, 2024, vol. 14, issue 7, 1-10

Abstract: The purpose of this study is to discover and excavate more key factors and signaling pathways that regulate the formation intensity of biofilms and to fully reveal the possible models affecting biofilm formation. By using gene homologous recombination and bioinformatics technology, a MutL protein-directed deletion mutant strain was successfully constructed. The growth status of the mutant strain was observed, and it was confirmed that, except for the change in cell morphology, there were no significant differences in growth and reproduction between the mutant strain and the wild-type strain. By using the induced biofilm formation technique, the significant decrease in biofilm formation in the MutL mutant strain was successfully verified. The plate confrontation test confirmed that the inhibitory ability of the mutant strain against rice blast fungus was not significantly different from that of the WT strain. The colonization ability of the mutant strain on rice stems was tested, and it was confirmed that the colonization ability of the mutant strain was significantly lower than that of the WT strain. In terms of the prevention and control effect of rice blast disease, the mutant strain showed a significant decrease. By using transcriptomic big data, the gene and pathway expression differences between the mutant strain and the WT strain during biofilm formation were analyzed. The analysis revealed no significant correlation with the previously reported spo0A and tapA-sipW-tasA pathways. The key factor capB of the polyglutamic acid signaling pathway, which affects the formation of the biological model, was found to have a significant decrease in expression. A mechanical hypothesis was proposed: MutL may participate in regulating the formation intensity of Bacillus biofilms by regulating the formation of glutamic acid to polyglutamic acid.

Keywords: B. amyloliquefaciens; biofilm formation; mechanism (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/14/7/1193/pdf (application/pdf)
https://www.mdpi.com/2077-0472/14/7/1193/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:14:y:2024:i:7:p:1193-:d:1438940

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().