Analysis of BnGPAT9 Gene Expression Patterns in Brassica napus and Its Impact on Seed Oil Content

Man Xing, Bo Hong, Mengjie Lv, Xueyi Lan, Danhui Zhang, Chunlei Shu, Shucheng Qi, Zechuan Peng, Chunyun Guan, Xinghua Xiong and Luyao Huang ()
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Man Xing: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Bo Hong: College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Mengjie Lv: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Xueyi Lan: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Danhui Zhang: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Chunlei Shu: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Shucheng Qi: College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Zechuan Peng: College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Chunyun Guan: College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Xinghua Xiong: College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Luyao Huang: Yichun Academy of Sciences, Yichun 336000, China

Agriculture, 2024, vol. 14, issue 8, 1-18

Abstract: Glycerol-3-phosphate acyltransferase ( GPAT ) genes encode enzymes involved in the biosynthesis of plant oils. Rapeseed has four BnGPAT9 genes, but the expression patterns and functions of these four homologous copies in rapeseed for seed oil accumulation are not well understood. In this study, we cloned the four BnGPAT9 genes and their promoters from Brassica napus and found significant differences in the expression of BnGPAT9 genes among different rapeseed varieties. We confirmed that BnGPAT9-A01/C01 are highly conserved in rapeseed, with high expression levels in various tissues, especially during the late stages of silique development and seed maturation. All four BnGPAT9 genes ( BnGPAT9-A01/C01/A10/C09 ) can promote seed oil accumulation, but BnGPAT9-A01/C01 have a greater effect. Overexpression in Arabidopsis and rapeseed increased seed oil content and altered fatty acid composition, significantly increasing linolenic acid content. Transcriptome analysis revealed that BnGPAT9 genes promote the upregulation of genes related to oil synthesis, particularly those in the Plant–pathogen interaction, alpha-Linolenic acid metabolism, MAPK signaling pathway—plant, and Glutathione metabolism pathways. In summary, these results indicate that the four BnGPAT9 genes in rapeseed have different expression patterns and roles in regulating seed oil accumulation, with BnGPAT9-A01/C01 contributing the most to promoting oil accumulation.

Keywords: Brassica napus L.; BnGPAT9; oil content; fatty acids (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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