PlLAC15 Facilitates Syringyl Lignin Deposition to Enhance Stem Strength in Herbaceous Peony

Yuehan Yin, Shiqi Zuo, Minghao Zhao, Jun Tao, Daqiu Zhao and Yuhan Tang ()
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Yuehan Yin: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Shiqi Zuo: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Minghao Zhao: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Jun Tao: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Daqiu Zhao: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Yuhan Tang: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China

Agriculture, 2024, vol. 14, issue 9, 1-13

Abstract: Stems are prone to bending or lodging due to inadequate stem strength, which seriously reduces the cut-flower ornamental quality of herbaceous peony ( Paeonia lactiflora Pall.). Plant LACCASE (LAC), a copper-containing polyphenol oxidase, has been shown to participate in the polymerization process of monolignols; however, the role of LAC in regulating the stem strength of P. lactiflora remains unclear. Here, the full-length cDNA of PlLAC15 , which demonstrated a positive association with stem strength, was isolated. It consisted of 1790 nucleotides, encoding 565 amino acids that had four typical laccase copper ion-binding domains. Moreover, PlLAC15 was highly expressed in the stem, and its expression level gradually significantly increased during stem development. Furthermore, PlLAC15 was found to be localized specifically to the cell wall, and its recombinant protein exhibited laccase activity. Additionally, the role of PlLAC15 in regulating the stem strength of P. lactiflora was confirmed by transgenic studies. When PlLAC15 was overexpressed in tobacco, stem strength increased by more than 50%, S-lignin was significantly deposited, and the lignification degree of stem xylem fiber cells increased. These results suggested that PlLAC15 facilitated S-lignin deposition to enhance stem strength in P. lactiflora , which would provide precious information that benefits future exploration of stem bending or lodging resistance in plants.

Keywords: laccase; stem strength; syringyl lignin; herbaceous peony (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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