Identification of Hotspot Regions for Candidate Genes Associated with Peanut ( Arachis hypogaea L.) Pod and Seed Size on Chromosome A05

Xiaoji Zhang, Luhuan Wang, Qimei Liu, Xiaoyu Zhang, Yuexia Tian, Yunyun Xue, Huiqi Zhang, Na Li, Xin Zhang () and Dongmei Bai ()
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Xiaoji Zhang: College of Agronomy, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Luhuan Wang: College of Agronomy, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Qimei Liu: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Xiaoyu Zhang: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Yuexia Tian: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Yunyun Xue: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Huiqi Zhang: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Na Li: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Xin Zhang: Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China
Dongmei Bai: College of Agronomy, Shanxi Agricultural University, Taigu, Jinzhong 030801, China

Agriculture, 2024, vol. 14, issue 9, 1-16

Abstract: The size of peanut pods and seeds, which directly affects yield and quality, also has significant implications for mechanized production and market efficiency. Identifying relevant loci and mining candidate genes is crucial for cultivating high-yield peanut varieties. In this study, we employed advanced generation recombinant inbred lines developed by crossbreeding Huayu 44 and DF12 as the experimental material. Quantitative trait locus (QTL) mapping for traits related to pod and seed size was conducted across six environments. A total of 44 QTLs were detected, distributed on chromosomes A02, A05, B04, B08, and B10. An enrichment region for multiple QTLs was also identified on chromosome A05 (19.28~52.32 cm). In this region, 10 KASP markers were developed, narrowing the enrichment area to two candidate gene hotspot regions of 600.9 kb and 721.2 kb. By combining gene prediction and functional annotation within the intervals, 10 candidate genes, including those encoding cytochrome P450 protein, polyamine synthase, mannose-1-phosphate guanylyltransferase, pentatricopeptide repeat protein, and E2F transcription factor, were identified as regulators of pod and seed size. This study provides technical support for the genetic improvement and key gene identification of peanut pod and seed size.

Keywords: candidate genes; QTL mapping; whole-genome re-sequencing (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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