The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants

Wanqing Tan, Zhiyuan Wang and Liezhao Liu ()
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Wanqing Tan: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Zhiyuan Wang: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Liezhao Liu: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China

Agriculture, 2024, vol. 15, issue 1, 1-32

Abstract: The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeting locations, and the optimization of CRISPR vectors. In this review, we systematically describe the Class II Cas proteins that have been used in plants, deactivated Cas9 (dCas9) and its role in transcriptional regulation, precision editing systems, Cas9 protein variants, as well as methods and examples of CRISPR–Cas systems targeting various regions with different breadths. In addition, we outline the optimization plans based on CRISPR constructs that can overcome the pleiotropy of genes or accelerate the generation of transgene-free plants and the applications of CRISPR systems in plant breeding practices. Finally, we discuss the theory and development of “CRISPR plus”, and the integrated application of existing systems in more species.

Keywords: CRISPR–Cas; precise editing; CRISPR screen; transgene-free; crop improvement (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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