Combined Genomic and Transcriptomic Screening of Candidate Genes for Asymmetric Oviduct Development in Hens

Ruijie Li, Xiang Song, Xiao Lin, Mingli Deng, Yin Liu, Tuoyu Geng, Daoqing Gong, Minmeng Zhao and Long Liu ()
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Ruijie Li: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xiang Song: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xiao Lin: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Mingli Deng: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yin Liu: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Tuoyu Geng: Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
Daoqing Gong: Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
Minmeng Zhao: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Long Liu: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Agriculture, 2025, vol. 15, issue 12, 1-25

Abstract: Background: While the mechanism of asymmetric gonadal development is generally understood, the mechanism of asymmetric oviduct development remains unclear. Methods: Right and left oviducts were collected from chick embryos at three developmental stages (Embryonic day 7.5, E9.5, and E11.5) for RNA-seq analysis (RNA-seq). Whole-genome resequencing (WGRS) was performed on hens with bilateral reproductive systems (a rare natural occurrence) and unilateral controls. These data were co-analyzed with public RNA-seq data of female embryonic gonads at different developmental stages (E4.5, E5.5, and E6.5) to screen for candidate genes affecting oviduct degeneration/development. Results: RNA-seq analyses showed that a total of 27, 10, and 38 DEGs were identified between the left and right oviducts at E7.5, E9.5, and E11.5, respectively. WGRS analyses revealed 1045 differentially mutated genes (DMGs) between bilateral (D) and unilateral (S) groups. Preliminary validation highlighted BMP7 , PAK3 , SLC6A11 , PITX2 , and SMC1B as candidate genes influencing oviduct asymmetry. Conclusions: This study provides insights into the genetic basis of asymmetric oviduct development and lays the groundwork for breeding hens with bilateral reproductive systems.

Keywords: oviduct; degeneration; RNA-seq; left-right lateral asymmetry; WGRS (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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