Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease

Xinchun Liu, Yan Wang, Yating Zhang, Jingzhao Xia, Chenxi Liu, Yu Song, Tao Han, Songhong Wei () and Wenjing Zheng ()
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Xinchun Liu: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Yan Wang: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Yating Zhang: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Jingzhao Xia: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Chenxi Liu: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Yu Song: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Tao Han: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Songhong Wei: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Wenjing Zheng: Liaoning Rice Research Institute, Shenyang 110101, China

Agriculture, 2025, vol. 15, issue 12, 1-14

Abstract: Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensitivity. This research uses LAMP to develop rapid RBD pathogen detection systems. Primers were designed targeting the NRPS31 gene of Fusarium fujikuroi and conserved TEF1α sequences of Fusarium asiaticum , Fusarium proliferatum , and Fusarium andiyazi . These reactions at 60 °C for 60 min had a detection limit of 100 pg·μL −1 , and LAMP proved applicable in field trials.

Keywords: rice bakanae disease; loop-mediated isothermal amplification; pathogen detection; seed detection (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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