Bioactive Compounds, Ruminal Fermentation, and Anthelmintic Activity of Specialty Coffee and Spent Coffee Grounds In Vitro

Matej Leško, Daniel Petrič, Matúš Várady, Pola Sidoruk, Robert Mikula, Sylwester Ślusarczyk, Paweł Edward Hodurek, Michaela Komáromyová, Michal Babják, Marián Várady, Amlan Kumar Patra, Adam Cieslak and Zora Váradyová ()
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Matej Leško: Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia
Daniel Petrič: Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia
Matúš Várady: Department of Food Hygiene, Technology and Safety, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia
Pola Sidoruk: Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland
Robert Mikula: Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland
Sylwester Ślusarczyk: Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland
Paweł Edward Hodurek: Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland
Michaela Komáromyová: Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia
Michal Babják: Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia
Marián Várady: Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia
Amlan Kumar Patra: American Institute for Goat Research, Langston University, 100 Success Ave, Langston, OK 73050, USA
Adam Cieslak: Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland
Zora Váradyová: Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia

Agriculture, 2025, vol. 15, issue 14, 1-15

Abstract: We quantified the bioactive compounds of Ethiopian coffee (ETH), spent coffee grounds SCGs from ETH (SCG-ETH), and mixed SCGs (SCG-MIX) prepared by filtration methods and investigated the effect of SCG-ETH on ruminal fermentation as well as the anthelmintic activity of ETH. Three substrates, meadow hay (MH)-barley grain (MH-BG), MH-SCG-ETH, and BG-SCG-ETH (1:1 w / w ), were fermented using an in vitro gas production technique. The bioactive compounds were quantitatively analyzed using ultra-high-resolution mass spectrometry. We performed an in vitro larval development test to determine the anthelmintic effect of an aqueous extract of ETH against the gastrointestinal nematode (GIN) Haemonchus contortus . The total content of bioactive compounds was highest in SCG-ETH, followed by SCG-MIX and ETH (35.2, 31.2, and 20.9 mg/g dry matter, respectively). Total gas and methane production ( p < 0.001) were decreased by both MH-SCG-ETH and BG-SCG-ETH. The in vitro digestibility of dry matter was higher for MH-SCG-ETH and BG-SCG-ETH than for MH-BG. The aqueous ETH extract exhibited a strong larvicidal effect, with a mean lethal dose of 13.2 mg/mL for 50% mortality and 31.9 mg/L for 99% mortality. SCG substrates have the potential to modulate ruminal fermentation and serve as a source of anthelmintic bioactive compounds against GINs in ruminants.

Keywords: filtered specialty coffees; coffee grounds; anthelmintic compounds; methane emissions; ruminal fermentation; ciliate protozoan; bioactive substances (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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