A Seed Vigor Test Through a Biospeckle Laser: A Comparison of Local and Global Analyses

Bruno Vicentini, Roberto Alves Braga (), José Luís Contado, José Eduardo da Silva Gomes and Rolando de Jesus Gonzalez-Peña
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Bruno Vicentini: Departament of Automatica (DAT), School of Engineering, Federal University of Lavras (UFLA), Lavras 37203202, MG, Brazil
Roberto Alves Braga: Departament of Automatica (DAT), School of Engineering, Federal University of Lavras (UFLA), Lavras 37203202, MG, Brazil
José Luís Contado: Departament of Food Science (DCA), Federal University of Lavras (UFLA), Lavras 37203202, MG, Brazil
José Eduardo da Silva Gomes: Mechatronics Department (DMCVG), Federal Centre for Technological Education of Minas Gerais (CEFET-MG), Varginha 37203202, MG, Brazil
Rolando de Jesus Gonzalez-Peña: Department Fisiologia, Unidad de Biofísica y Física Médica, Facultad de Medicina y Odontología, Universidad de Valencia, 46010 Valencia, Spain

Agriculture, 2025, vol. 15, issue 14, 1-14

Abstract: Seed vigor testing traditionally requires large sample sizes and extended durations. The biospeckle laser (BSL) technique offers a faster, image-based alternative for seed analysis though the standardization of set protocols. This study evaluated the efficiency of local and global BSL analyses in bean seeds ( Phaseolus vulgaris L.). Two groups of seeds (872 in total) were classified into high- and low-vigor seeds using the emergence test over 800 samples. The BSL test was then applied to 72 seeds (36 per group), analyzing biological activity locally (vascular and embryonic areas) and globally (whole image). BSL analysis detected significant differences between the groups ( p < 0.05). Among the methods, the local analysis of the embryonic axis was most effective (F = 44.252, p = 0.000), showing a clearer distinction than the global analysis (F = 19.484, p = 0.000). The vascular area analysis did not yield significant results. These findings highlight the efficiency of the local BSL analysis at the embryonic axis for vigor tests compared to the global analysis. However, it was observed that the selected point in the local analysis affects the reliability of the vigor test. It was a relevant step toward standardization demanding additional tests in other species and varieties.

Keywords: dynamic laser speckle; seed analysis; laser speckle imaging (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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