Genome-Wide Identification and Cold Stress Response Analysis of the Rboh Gene Family in Pomegranate ( Punica granatum L.)

Yu Sheng, Xiaoyu Wang, Chenyu Wang, Xiaoyong Xu and Lijuan Jiang ()
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Yu Sheng: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Xiaoyu Wang: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Chenyu Wang: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Xiaoyong Xu: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
Lijuan Jiang: College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China

Agriculture, 2025, vol. 15, issue 17, 1-17

Abstract: Plant respiratory burst oxidase homolog ( Rboh ) genes are integral to the production of reactive oxygen species (ROS) and the regulation of stress responses. Here, bioinformatic techniques were employed to identify eight PgRboh genes ( PgRbohA–H ) in the genome of pomegranate ( Punica granatum L.) and conduct a systematic analysis of this family. The findings showed that all PgRbohs proteins possess characteristic NADPH oxidase domains and are predicted to be localized on the cell membrane. Experimental verification confirmed the membrane localization of PgRbohD and PgRbohE proteins. Phylogenetic analysis categorized the PgRbohs proteins into six distinct groups, suggesting potential functional divergence among these groups. Promoter analysis revealed a significant presence of cis-acting elements responsive to low-temperature and methyl jasmonate (MeJA). The expression of PgRboh genes was found to be tissue-specific. Additionally, real-time PCR (RT-qPCR) was used to analyze expression patterns in response to low-temperature stress that involves multiple PgRboh genes in the cold response process. Overall, our results lay an important foundation for subsequent studies on the cold resistance function of pomegranate Rboh genes and provides new ideas for the breeding of new cold-resistant pomegranate varieties.

Keywords: bioinformatics analysis; low-temperature stress; pomegranate; Rboh gene family (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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