Effects of Cellulase and Lactiplantibacillus plantarum on Chemical Composition, Fermentation Characteristics, and Bacterial Community of Pennisetum giganteum z.x.lin Silage

Zhili Wu, Xiongfei Zhang, Rongnuo Li, Jingtao Hui, Lu Deng, Inho Kim, Jie Wei, Junhu Yao and Xinjian Lei ()
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Zhili Wu: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Xiongfei Zhang: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Rongnuo Li: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Jingtao Hui: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Lu Deng: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Inho Kim: Department of Animal Resource and Science, Dankook University, Cheonan 31116, Republic of Korea
Jie Wei: Xi’an Architectural Design and Research Institute Co., Ltd., Xi’an 710000, China
Junhu Yao: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Xinjian Lei: College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China

Agriculture, 2025, vol. 15, issue 1, 1-16

Abstract: In order to explore the effects of additives on the chemical composition, fermentation characteristics, and bacterial community of Pennisetum giganteum z.x.lin silage, Pennisetum giganteum z.x.lin was ensiled with no additives (CON), cellulase (CE), Lactiplantibacillus plantarum (LP), or the combination of cellulase and Lactiplantibacillus plantarum (LPCE) at room temperature for 60 days, respectively. The results indicated that LPCE had the highest dry matter (DM) content. Compared with CON, LP exhibited higher ( p < 0.05) levels of water-soluble carbohydrate (WSC), crude protein (CP), and lactic acid (LA), along with a higher ( p < 0.05) ratio of LA/acetic acid (AA). Meanwhile, silage inoculated with cellulase (CE and LPCE) showed lower ( p < 0.05) contents of acid detergent fiber (ADF) and neutral detergent fiber (NDF) than CON. Furthermore, additive treatments improved the bacterial community composition of silage, and Lactobacillus was abundant in LPCE (LDA score > 4.0). Compared with CE and LP, LPCE more effectively promoted the transformation of microbial functions, resulting in an upregulated ( p < 0.05) carbohydrate metabolism and a downregulated ( p < 0.05) membrane transport. In conclusion, cellulase or Lactiplantibacillus plantarum improved the silage quality of Pennisetum giganteum z.x.lin by reducing the fiber content or enhancing LA fermentation, and their combination exhibited a powerful ability to establish a bacterial community dominated by Lactobacillus , which facilitated the production of high-quality silage.

Keywords: bio-additives; ensiling; enzyme-bacteria synergy; microbial diversity; Pennisetum giganteum z.x.lin (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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