A 7-bp Insertion/Deletion Functional Variation in Fatty Acid Synthase Gene Is Associated with Abdominal Fat Accumulation

Meng Yuan, Yaqian Han, Xinyi Yin, Xin Liu, Bohan Cheng, Ning Wang, Hui Li, Zhiping Cao, Wenpeng Zhang and Shouzhi Wang ()
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Meng Yuan: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Yaqian Han: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Xinyi Yin: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Xin Liu: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Bohan Cheng: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Ning Wang: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Hui Li: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Zhiping Cao: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Wenpeng Zhang: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China
Shouzhi Wang: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, China

Agriculture, 2025, vol. 15, issue 22, 1-12

Abstract: Reducing abdominal fat (AF) in broilers is a key objective in broiler breeding, as excessive AF deposition has an unfavorable effect on reproductive and growth traits. Fatty acid synthase gene ( FASN ) encodes the fatty acid synthase, which promotes fatty acid synthesis and lipid synthesis. Our previous study identified a 7-bp InDel variant (g.4944396_4944402delGACTTCA, rs1060467633) in intron 42 of chicken FASN , demonstrating a marked association with chicken AF. To investigate the underlying mechanism of this association, we conducted dual-luciferase reporter assay, bioinformatics analysis and electrophoretic mobility shift assay (EMSA). Luciferase reporter showed that the deletion allele had significantly higher activity than the insertion allele ( p < 0.05), indicating its potential role in regulating gene expression. Bioinformatics analysis and EMSA indicated that the insertion allele created binding site for cAMP-responsive element-binding protein 1 (CREB1). Luciferase reporter confirmed that the insertion allele suppresses FASN expression through CREB1, with a stronger inhibitory effect than the deletion allele. In conclusion, rs1060467633, as a functional variant, regulates FASN expression and AF deposition by altering the binding to CREB1 in chickens.

Keywords: chicken; belly fat; FASN; CREB1; functional mutation (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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