Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages

Guilherme Medeiros Leite, Edson Mauro Santos, Juliana Silva de Oliveira, Danillo Marte Pereira (), Celso José Bruno de Oliveira, Jorge Vinicius Fernandes Lima Cavalcanti, Vanessa Maria Rodrigues de Lima, João Paulo Vieira de Melo Fernandes de Lima, Paloma Gabriela Batista Gomes, Ricardo Loiola Edvan, Rafael de Souza Miranda, Daniele de Jesus Ferreira, Fagton de Mattos Negrão and Anderson de Moura Zanine
Additional contact information
Guilherme Medeiros Leite: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Edson Mauro Santos: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Juliana Silva de Oliveira: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Danillo Marte Pereira: Department of Animal Science, Federal University of Maranhão, Chapadinha 65500-000, MA, Brazil
Celso José Bruno de Oliveira: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Jorge Vinicius Fernandes Lima Cavalcanti: Department of Chemical Engineering, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
Vanessa Maria Rodrigues de Lima: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
João Paulo Vieira de Melo Fernandes de Lima: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Paloma Gabriela Batista Gomes: Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
Ricardo Loiola Edvan: Department of Animal Science, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Rafael de Souza Miranda: Department of Chemical Engineering, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
Daniele de Jesus Ferreira: Department of Animal Science, Federal University of Maranhão, Chapadinha 65500-000, MA, Brazil
Fagton de Mattos Negrão: Department of Animal Science, Federal Institute of Education, Science, and Technology of Rondônia, Colorado do Oeste 76993-000, RO, Brazil
Anderson de Moura Zanine: Department of Animal Science, Federal University of Maranhão, Chapadinha 65500-000, MA, Brazil

Agriculture, 2025, vol. 15, issue 3, 1-25

Abstract: This study aimed to isolate, characterize, and identify acetic acid-producing lactic acid bacteria from fresh sorghum plants and silage, and to evaluate the effect of the isolates as microbial inoculants on taxonomic diversity and silage fermentation quality. For the first experimental stage, eight experimental silos were prepared, and the fresh sorghum plant cv. BRS Ponta Negra ( Sorghum bicolor (L.) Moench.) was sampled to characterize and identify the bacteria. Five strains were chosen to be inoculated in the second experimental stage, in a 7 × 2 factorial design, with seven treatments and two opening times, in four replications. Four types of species were identified, with Lactiplantibacillus plantarum predominating at 72.73%. There was an interaction effect between treatments and opening times on effluent losses, gas losses, the population of lactic acid bacteria, yeasts, and lactic acid content. The aerobic stability treatments that stood out were Lactiplantibacillus plantarum (GML 66) and Weissella cibaria , which showed 71.75 and 68.87 h of stability. The use of Lactiplantibacillus plantarum (GML 66) as a microbial inoculant in sorghum silage increased the dry matter content, reduced effluent losses, and improved dry matter recovery. It also reduced the yeast population in the silage, promoting greater aerobic stability in the silage.

Keywords: acetic acid; aerobic stability; forage conservation; Lactiplantibacillus plantarum; fermentation quality (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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