The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens

Paola Aparecida Damázio Rodrigues (), Joyce Andrade da Silva, José Cavalcante Souza Vieira, Gabrieli Andressa de Lima, Laís Garcia Cordeiro, Elis Omar Figueroa Castillo, Júlia de Lima Lopes, Marília Afonso Rabelo Buzalaf, Pedro de Magalhães Padilha and José Roberto Sartori
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Paola Aparecida Damázio Rodrigues: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
Joyce Andrade da Silva: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
José Cavalcante Souza Vieira: Department of Chemistry and Biochemistry, Institute of Biosciences (IBB), São Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil
Gabrieli Andressa de Lima: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
Laís Garcia Cordeiro: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
Elis Omar Figueroa Castillo: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
Júlia de Lima Lopes: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil
Marília Afonso Rabelo Buzalaf: Department of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru 17012-901, SP, Brazil
Pedro de Magalhães Padilha: Department of Chemistry and Biochemistry, Institute of Biosciences (IBB), São Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil
José Roberto Sartori: Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil

Agriculture, 2025, vol. 15, issue 8, 1-16

Abstract: Pequi oil (PO) is a natural feed additive rich in bioactive compounds, which can modulate antioxidant and immunological systems. Thus, the aim of this study was to evaluate the proteomic profile of laying hens supplemented with PO under heat stress conditions. Ninety-six 26-week-old Lohmann White hens were housed in a completely randomized design with a 2 × 2 factorial arrangement, with two climate chambers (cyclic heat stress and thermoneutral) and two diets (control and 0.6% PO). At 38 weeks old, liver samples were collected for protein extraction and digestion, and were submitted to liquid chromatography–tandem mass spectrometry (LC-MS/MS). A total of 996 differentially expressed proteins were identified in the liver proteome of laying hens fed with 0.6% PO under heat stress. These upregulated proteins (0.95 ≤ p ≤ 1.00) are associated with lipid metabolism (apolipoprotein B; vitellogenin-1; ovotransferrin), the antioxidant system (protein disulfide-isomerase A4; superoxide dismutase 1_ soluble; catalase), the immune system (Ig-like domain-containing protein) and chaperones (HSP 90; HSP 70). PO positively modulates a network of heat shock proteins and antioxidant enzymes, and the unique proteins identified can contribute to the discovery of new biomarkers related to heat stress reduction by phytogenic additives.

Keywords: antioxidant; functional additive; oxidative stress; poultry; proteomic; shotgun (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2025
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