Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops

Joel P. Hague, Stephen L. Dellaporta, Maria A. Moreno, Chip Longo, Kimberly Nelson and Albert P. Kausch
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Joel P. Hague: Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA
Stephen L. Dellaporta: Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
Maria A. Moreno: Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
Chip Longo: Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA
Kimberly Nelson: Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA
Albert P. Kausch: Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA

Agriculture, 2012, vol. 2, issue 4, 1-21

Abstract: Advanced genetic and biotechnology tools will be required to realize the full potential of food and bioenergy crops. Given current regulatory concerns, many transgenic traits might never be deregulated for commercial release without a robust gene confinement strategy in place. The potential for transgene flow from genetically modified (GM) crops is widely known. Pollen-mediated transfer is a major component of gene flow in flowering plants and therefore a potential avenue for the escape of transgenes from GM crops. One approach for preventing and/or mitigating transgene flow is the production of trait linked pollen sterility. To evaluate the feasibility of generating pollen sterility lines for gene confinement and breeding purposes we tested the utility of a promoter ( Zm13 Pro) from a maize pollen-specific gene ( Zm13 ) for driving expression of the reporter gene GUS and the cytotoxic gene barnase in transgenic rice ( Oryza sativa ssp. Japonica cv. Nipponbare) as a monocot proxy for bioenergy grasses. This study demonstrates that the Zm13 promoter can drive pollen-specific expression in stably transformed rice and may be useful for gametophytic transgene confinement and breeding strategies by pollen sterility in food and bioenergy crops.

Keywords: bioenergy; gene confinement; GM crops; transgenic plants; pollen sterility; regulatory concerns; agricultural regulation; environmental regulation; gametophyte; Oryza sativa (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2012
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