Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry

Joseph Minicozzi, Susan Sanchez, Margie D. Lee, Peter S. Holt, Charles L. Hofacre and John J. Maurer
Additional contact information
Joseph Minicozzi: Department of Population Health, The University of Georgia, 953 College Station Rd., Athens, GA 30602, USA
Susan Sanchez: Department of Infectious Diseases, The University of Georgia, Athens, GA 30602, USA
Margie D. Lee: Department of Population Health, The University of Georgia, 953 College Station Rd., Athens, GA 30602, USA
Peter S. Holt: Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA
Charles L. Hofacre: Department of Population Health, The University of Georgia, 953 College Station Rd., Athens, GA 30602, USA
John J. Maurer: Department of Population Health, The University of Georgia, 953 College Station Rd., Athens, GA 30602, USA

Agriculture, 2013, vol. 3, issue 3, 1-17

Abstract: Accurate and fast detection of harmful Salmonella is a major concern of food safety. Common Salmonella serotypes responsible for human associated foodborne outbreaks are S. Enteritidis, S. Hadar, S. Heidelberg, and S. Typhimurium are also commonly isolated from poultry. Serology is commonly used to monitor disease in poultry, therefore application of Salmonella serotype-specific test will have added value in Salmonella surveillance or monitoring vaccine efficacy. Recombinant flagellins were purified to be used as antigens in an ELISA. In this study, an ELISA was developed for the serological detection of S. Enteritidis. Once optimized, 500 ng of purified recombinant S. Enteritidis flagellin and a 1:64 dilution were determined to be optimal for testing sera. A negative baseline cutoff was calculated to be an optical density (OD) of 0.35. All sera from birds with history of S. Enteritidis exposure tested positive and all sera from chickens with no exposure tested negative to this Salmonella serotype. Current ELISA for serological detection of Salmonella suffers from cross reactivity inherent in lipopolysaccharide (LPS) or whole cell antigen based serological tests. This new ELISA eliminates common cross reactivity by focusing specifically on the flagellins of the Salmonella serotypes common in poultry and associated with foodborne outbreaks.

Keywords: Salmonella; ELISA; poultry; serotype; Enteritidis (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2013
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