Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150

Lihua Li, Jincai Ma, A. Mark Ibekwe, Qi Wang and Ching-Hong Yang
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Lihua Li: The MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Jincai Ma: College of Environment and Resources, Jilin University, Changchun, Jilin 130021, China
A. Mark Ibekwe: USDA-ARS, U.S. Salinity Laboratory, 450 W. Big Springs Rd, Riverside, CA 92507, USA
Qi Wang: The MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Ching-Hong Yang: Department of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211, USA

Agriculture, 2015, vol. 6, issue 1, 1-15

Abstract: Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain B068150 inoculated in a greenhouse for 90 days, and the colonization ability of strain B068150 in cucumber rhizosphere and non-rhizosphere soils was determined. Changes in total bacteria and fungi community composition and structures using denaturing gradient gel electrophoresis (DGGE) and sequencing were determined. Colony counts showed that B068150 colonization in the rhizosphere was significantly higher ( p < 0.001) than in non-rhizosphere soils. Based on our data, the introduction of B. bacillus B068150 did not change the diversity of microbial communities significantly in the rhizosphere of three soils. Our data showed that population density of B068150 in clay soil had a significant negative correlation on bacterial diversity in cucumber rhizosphere in comparison to loam and sandy soils, suggesting that the impact of B068150 might be soil specific.

Keywords: cucumber; Bacillus subtilis; Microbial community; colonization; diversity (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2015
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