The Influence of Seed Viability on the Germination and In Vitro Multiple Shoot Regeneration of Soybean ( Glycine max L.)

Phetole Mangena and Phatlane William Mokwala
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Phetole Mangena: Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa
Phatlane William Mokwala: Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa

Agriculture, 2019, vol. 9, issue 2, 1-12

Abstract: The moisture status of seeds is usually high during the period of harvest and deterioration (loss of viability) starts to occur when seeds are stored for longer periods. In the present study, soybean seeds were evaluated using a standard germination test, in vitro germination, and for efficient multiple shoot induction, following storage under ambient conditions for 0, 3, 6 and 9 months. Results showed that seeds stored for more than 3 months had reduced moisture content and decreased germination percentages in LS677, LS678, Dundee, Peking, TGx1740-2F and TGx1835-10E of the tested genotypes. In particular, seeds stored for 9 months showed significantly poor seed viability and less than 50% overall seed germination (Dundee—42%, LS678—49%, TGx 1740-2F—44%, TGx 1835-10E—48%), except for LS677 and Peking, with 52 and 55%, respectively. The efficiency of multiple shoot induction also decreased with prolonged seed storage, with all genotypes recording an overall decline from about 96% to 40% regeneration efficiency within 9 months. The results obtained clearly indicated that high germination rates and efficient in vitro shoot induction depended largely on seed viability and storage duration, and significantly differed according to genotypes.

Keywords: germination; seed storage; seed viability; seedling vigour; shoot multiplication; soybean (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2019
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