Selective modulation of protein kinase C-Θ during T-cell activation

Colin R. F. Monks, Hannah Kupfer, Idan Tamir, Avlin Barlow and Abraham Kupfer
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Colin R. F. Monks: National Jewish Center for Immunology
Hannah Kupfer: National Jewish Center for Immunology
Idan Tamir: National Jewish Center for Immunology
Avlin Barlow: Yale University School of Medicine
Abraham Kupfer: National Jewish Center for Immunology

Nature, 1997, vol. 385, issue 6611, 83-86

Abstract: Abstract EVERY cell contains many families of protein kinases, and may express several structurally related yet genetically distinct kinases of each family. The activity of the serine/threonine protein kinase C (PKC) enzymes1–2 has long been implicated in T-cell activation3, but it is not known which members of the PKC family regulate the T-cell response to foreign antigens. The activation of T cells by antigen-presenting cells (APCs) is spatially restricted to their site of contact, where receptors on the T cells engage their counter-receptors on the APCs4,5. We used this localized engagement to identify, at the single-cell level, intracel-lular proteins involved in the activation process. By digital immunofluorescence microscopy, we localized six isoforms of PKC in antigen-specific T-cell clones activated by APCs. Surprisingly, only PKC-Θ translocated to the site of cell contact. Accordingly, in vitro kinase activity assays of PKC immunoprecipitates from the conjugates of T cells and APCs showed a selective increase in the activity of PKC-Θ, indicating that the translocated enzyme is active. Several modes of partial T-cell activation that failed to cause PKC-Θ translocation also failed to cause T-cell proliferation, further suggesting the involvement of PKC-Θ in T-cell activation.

Date: 1997
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DOI: 10.1038/385083a0

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