The C-terminal domain of RNA polymerase II couples mRNA processing to transcription

Susan McCracken, Nova Fong, Krassimir Yankulov, Scott Ballantyne, Guohua Pan, Jack Greenblatt, Scott D. Patterson, Marvin Wickens and David L. Bentley
Additional contact information
Susan McCracken: Amgen Institute
Nova Fong: Amgen Institute
Krassimir Yankulov: Amgen Institute
Scott Ballantyne: Amgen Institute
Guohua Pan: Amgen Institute
Jack Greenblatt: Amgen Institute
Scott D. Patterson: Amgen Institute
Marvin Wickens: Amgen Institute
David L. Bentley: Amgen Institute

Nature, 1997, vol. 385, issue 6614, 357-361

Abstract: Abstract Messenger RNA is produced by RNA polymerase II (pol II) transcription, followed by processing of the primary transcript. Transcription, splicing and cleavage–polyadenylation can occur independently in vitro, but we demonstrate here that these processes are intimately linked in vivo. We show that the carboxy-terminal domain (CTD) of the pol II large subunit is required for efficient RNA processing. Splicing, processing of the 3′ end and termination of transcription downstream of the poly(A) site, are all inhibited by truncation of the CTD. We found that the cleavage–polyadenylation factors CPSF and CstF specifically bound to CTD affinity columns and copurified with pol II in a high-molecular-mass complex. Our demonstration of an association between the CTD and 3′-processing factors, considered together with reports of a similar interaction with splicing factors1,2, suggests that an mRNA 'factory' exists which carries out coupled transcription, splicing and cleavage–polyadenylation of mRNA precursors.

Date: 1997
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DOI: 10.1038/385357a0

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