Trans-activation of group II intron splicing by nuclear U5 snRNA

Martin Hetzer, Gabriele Wurzer, Rudolf J. Schweyen and Manfred W. Mueller
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Martin Hetzer: University of Vienna
Gabriele Wurzer: University of Vienna
Rudolf J. Schweyen: University of Vienna
Manfred W. Mueller: University of Vienna

Nature, 1997, vol. 386, issue 6623, 417-420

Abstract: Abstract Similarities between RNA splicing during autocatalytic excision of group II introns and pre-mRNA processing led to the hypothesis that group II introns might be the evolutionary predecessors of spliceosomal small nuclear RNAs1–4. The ID3 subdomain stem-loop structure of group II introns, the proposed analogue of the spliceosomal U5 snRNA5, is thought to be essential for 5′ splice site recognition and anchoring of the free 5′ exon6. Using the group II intron bII we have analysed the role of ID3 in splicing. In the absence of ID3 the 5′ splice site was recognized accurately and efficiently, but exon anchoring was greatly reduced. This step was restored in the presence of RNA fragments consisting of either the terminal stem–loop structure of ID3 or spliceosomal U5 snRNA. This suggests that the predominant role of both RNAs is to anchor the 5′ exon during exon ligation. Furthermore, as U5 complements for the loss of ID3, a similar network of structural RNAs may form the catalytic core of both group II introns and spliceosomes.

Date: 1997
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DOI: 10.1038/386417a0

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