Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic

Jaeseob Kim, Kirby Johnson, Hui Ju Chen, Sean Carroll and Allen Laughon ()
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Jaeseob Kim: *Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin
Kirby Johnson: 445 Henry Mall, University of Wisconsin-Madison
Hui Ju Chen: McArdle Laboratory, University of Wisconsin-Madison
Sean Carroll: *Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin
Allen Laughon: Correspondence and requests for materials should be addressed to A.L.

Nature, 1997, vol. 388, issue 6639, 304-308

Abstract: Abstract The TGF-β (transforming growth factor-β)-related signalling proteins, including Decapentaplegic (Dpp) in Drosophila and bone morphogenic proteins and activin in vertebrates, affect the growth and patterning of a great variety of structures. However, the mechanisms by which these ligands regulate gene expression are not understood. Activation of complexes of type I with type II receptors results in the phosphorylation and nuclear localization of members of the SMAD protein family1,2,3,4,5,6,7,8,9, which are thought to act as co-activators of transcription, perhaps in conjunction with sequence-specific cofactors10. Here we show that the amino-terminal domain of the Drosophila Mothers against dpp protein (Mad), a mediator of Dpp signalling11,12,13,14, possesses a sequence-specific DNA-binding activity that becomes apparent when carboxy-terminal residues are removed. Mad binds to and is required for the activation of an enhancer within the vestigial wing-patterning gene in cells across the entire developing wing blade. Mad also binds to Dpp-response elements in other genes. These results suggest that Dpp signalling regulates gene expression by activating Mad binding to target gene enhancers.

Date: 1997
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DOI: 10.1038/40906

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