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Calcium turns turquoise into gold

Tullio Pozzan ()
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Tullio Pozzan: CNR Centre of Biomembranes, University of Padova

Nature, 1997, vol. 388, issue 6645, 834-835

Abstract: The concentration of calcium in living cells can be measured using differently coloured green fluorescent protein (GFP) mutants. Two groups have independently linked blue-shifted GFP mutants to longer-wavelength GFP mutants through connecting sequences to which Ca2+-calmodulin or Ca2+ alone can bind. Fluorescence energy resonance transfer occurs between the two mutants, but it is very sensitive to the distance and orientation of the fluorophores, and so can detect Ca2+ binding.

Date: 1997
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DOI: 10.1038/42151

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