RETRACTED ARTICLE: Identification and role of adenylyl cyclase in auxin signalling in higher plants

Takanari Ichikawa, Yoshihito Suzuki, Inge Czaja, Carla Schommer, Angela LeBnick, Jeff Schell and Richard Walden ()
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Takanari Ichikawa: Max Planck Institut für Züchtungsforschung
Yoshihito Suzuki: University of Tokyo
Inge Czaja: Max Planck Institut für Züchtungsforschung
Carla Schommer: Max Planck Institut für Züchtungsforschung
Angela LeBnick: Max Planck Institut für Züchtungsforschung
Jeff Schell: Max Planck Institut für Züchtungsforschung
Richard Walden: Max Planck Institut für Züchtungsforschung

Nature, 1997, vol. 390, issue 6661, 698-701

Abstract: Abstract Cyclic AMP is an important signalling molecule in prokaryotes and eukaryotes1, but its significance in higher plants has been generally doubted2 because they have low adenylyl cyclase activity and barely detectable amounts of cAMP3. Here we used activation T-DNA tagging to create tobacco cell lines that can proliferate in the absence of the phytohormone auxin in the culture media4,5. The sequence tagged in one line, axi 141, was used to isolate a complementary DNA encoding adenylyl cyclase, the first from a higher plant. Sequence analysis reveals that the tobacco adenylyl cyclase is probably soluble, contains characteristic leucine-rich repeats, and bears similarity with adenylyl cyclase from the yeast Schizosaccharomyces pombe. Expression of the cDNA in Escherichia coli results in an increase in endogenous cAMP levels, and in yeast its expression functionally complements the cry1 mutation. Tobacco protoplasts treated with cAMP, or the adenylyl cyclase activator forskolin, no longer require auxin to divide. This finding, together with the observation that the adenylyl cyclase inhibitor dideoxyadenosine inhibits cell proliferation in the presence of auxin, suggests that cAMP is involved in auxin-triggered cell division in higher plants.

Date: 1997
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DOI: 10.1038/37810

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