Immunoreceptor DAP12 bearing a tyrosine-based activation motif is involved in activating NK cells

Lewis L. Lanier (), Brian C. Corliss, Jun Wu, Clement Leong and Joseph H. Phillips
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Lewis L. Lanier: DNAX Research Institute of Molecular and Cellular Biology
Brian C. Corliss: DNAX Research Institute of Molecular and Cellular Biology
Jun Wu: DNAX Research Institute of Molecular and Cellular Biology
Clement Leong: DNAX Research Institute of Molecular and Cellular Biology
Joseph H. Phillips: DNAX Research Institute of Molecular and Cellular Biology

Nature, 1998, vol. 391, issue 6668, 703-707

Abstract: Abstract Natural killer (NK) cells express cell-surface receptors of the immunoglobulin and C-type lectin superfamilies that recognize major histocompatibility complex (MHC) class I peptides and inhibit NK-cell-mediated cytotoxicity1. These inhibitory receptors possess ITIM sequences (for immunoreceptor tyrosine-based inhibitory motifs) in their cytoplasmic domains that recruit SH2-domain-containing protein tyrosine phosphatases, resulting in inactivation of NK cells2,3,4. Certain isoforms of these NK-cell receptors lack ITIM sequences and it has been proposed that these ‘non-inhibitory’ receptors may activate, rather than inhibit, NK cells4,5,6. Here we show that DAP12, a disulphide-bonded homodimer containing an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain, non-covalently associates with membrane glycoproteins of the killer-cell inhibitory receptor (KIR) family without an ITIM in their cytoplasmic domain. Crosslinking of KIR–DAP12 complexes results in cellular activation, as demonstrated by tyrosine phosphorylation of cellular proteins and upregulation of early-activation antigens. Phosphorylated DAP12 peptides bind ZAP-70 and Syk protein tyrosine kinases, suggesting that the activation pathway is similar to that of the T- and B-cell antigen receptors.

Date: 1998
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DOI: 10.1038/35642

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