Rad23 links DNA repair to the ubiquitin/proteasome pathway

Cherylene Schauber, Li Chen, Prasad Tongaonkar, Irving Vega, David Lambertson, Warren Potts and Kiran Madura ()
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Cherylene Schauber: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
Li Chen: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
Prasad Tongaonkar: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
Irving Vega: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
David Lambertson: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
Warren Potts: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey
Kiran Madura: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey

Nature, 1998, vol. 391, issue 6668, 715-718

Abstract: Abstract Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair1,2,3. A regulatory role has been proposed for Rad23 because rad23 mutants are sensitive to ultraviolet light but are still capable of incising damaged DNA4,5. Here we show that Rad23 interacts with the 26S proteasome through an amino-terminal ubiquitin-like domain (UbLR23). The carboxy terminus of Rad23 binds to the Rad4 DNA repair protein and creates a link between the DNA repair and proteasome pathways. The ultraviolet sensitivity caused by deletion of the UbLR23 domain may therefore arise from its inability to interact with the proteasome. The fusion proteins glutathione S-transferase (GST)–Rad23 and Rad4–haemagglutinin (HA), and the proteasome subunits Cim3 and Cim5, cofractionate through consecutive chromatography steps. The ubiquitin-like domain of human Rad23 (UbLHRB) also interacts with the human proteasome. These results demonstrate that ubiquitin-like domains (UbLs) represent a new class of proteasome-interacting motifs.

Date: 1998
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DOI: 10.1038/35661

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