Group II intron splicing in vivo by first-step hydrolysis

Mircea Podar, Vi T. Chu, Anna Marie Pyle and Philip S. Perlman ()
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Mircea Podar: University of Texas Southwestern Medical Center
Vi T. Chu: Columbia University College of Physicians and Surgeons
Anna Marie Pyle: Columbia University College of Physicians and Surgeons
Philip S. Perlman: University of Texas Southwestern Medical Center

Nature, 1998, vol. 391, issue 6670, 915-918

Abstract: Abstract Group I, group II and spliceosomal introns splice by two sequential transesterification reactions1. For both spliceosomal and group II introns, the first-step reaction occurs by nucleophilic attack on the 5′ splice junction by the 2′ hydroxyl of an internal adenosine, forming a 2′–5′ phosphodiester branch in the intron. The second reaction joins the two exons with a 3′–5′ phosphodiester bond and releases intron lariat. In vitro, group II introns can self-splice by an efficient alternative pathway in which the first-step reaction occurs by hydrolysis. The resulting linear splicing intermediate participates in normal second-step reactions, forming spliced exon and linear intron RNAs2,3. Here we show that the group II intron first-step hydrolysis reaction occurs in vivo in place of transesterification in the mitochondria of yeast strains containing branch-site mutations. As expected, the mutations block branching, but surprisingly still allow accurate splicing. This hydrolysis pathway may have been a step in the evolution of splicing mechanisms.

Date: 1998
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DOI: 10.1038/36142

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