Antagonism between extradenticle function and Hedgehog signalling in the developing limb

Sergio González-Crespo, Muna Abu-Shaar, Miguel Torres, Carlos Martínez-A, Richard S. Mann and Ginés Morata ()
Additional contact information
Sergio González-Crespo: Centro de Biología Molecular, CSIC-UAM, Universidad Autónoma de Madrid
Muna Abu-Shaar: Molecular & Biophysical Studies, Columbia University College of Physicians and Surgeons
Miguel Torres: Centro Nacional de Biotecnologia, Universidad Autónoma de Madrid
Carlos Martínez-A: Centro Nacional de Biotecnologia, Universidad Autónoma de Madrid
Richard S. Mann: Molecular & Biophysical Studies, Columbia University College of Physicians and Surgeons
Ginés Morata: Centro de Biología Molecular, CSIC-UAM, Universidad Autónoma de Madrid

Nature, 1998, vol. 394, issue 6689, 196-200

Abstract: Abstract The Drosophila homeobox gene extradenticle (exd) encodes a highly conserved cofactor of Hox proteins1,2,3. exd activity is regulated post-translationally by a mechanism involving nuclear translocation4,5; only nuclear Exd protein is functional. The exd gene is required for patterning of the proximal region of the leg6,7, whereas patterning of the distal region requires signalling by the Wingless (Wg) and Decapentaplegic (Dpp)8,9 proteins, which are in turn activated by Hedgehog (Hh)10. Here we show that exd function and Dpp/Wg signalling are antagonistic and divide the leg into two mutually exclusive domains. In the proximal domain, exd activity prevents cells from responding to Dpp and Wg. Conversely, in the distal domain, exd function is suppressed by the Dpp/Wg response gene Distal-less (Dll), which prevents the nuclear transport of Exd. We also found that the product of a murine homologue of exd (Pbx1) is regulated at the subcellular level, and that its pattern of nuclear localization in the mouse limb resembles that of Exd in the Drosophila leg. These findings suggest that the division of the limb into two antagonistic domains, as defined by exd (Pbx1) function and Hh signalling, may be a general feature of limb development.

Date: 1998
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/BF28197 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:394:y:1998:i:6689:d:10.1038_28197

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/28197

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().