DNA fingerprinting Dolly

Esther N. Signer (), Yuri E. Dubrova, Alec J. Jeffreys, Colin Wilde (), Lynn M. B. Finch, Michelle Wells and Malcolm Peaker
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Esther N. Signer: University of Leicester
Yuri E. Dubrova: University of Leicester
Alec J. Jeffreys: University of Leicester
Colin Wilde: Hannah Research Institute
Lynn M. B. Finch: Hannah Research Institute
Michelle Wells: Hannah Research Institute
Malcolm Peaker: Hannah Research Institute

Nature, 1998, vol. 394, issue 6691, 329-330

Abstract: Abstract The birth of Dolly1 has raised considerable interest and debate over the potential for cloning mammals, including humans. However, there are concerns about the authenticity of Dolly, and whether she could have been derived not from an adult donor mammary cell, but instead from a contaminating sheep cell culture or from a fetal cell present in the udder of the pregnant ewe donor2. Microsatellite typing suggested but did not prove authenticity1,2. We have therefore carried out a DNA fingerprint analysis to determine the origin of the donor cell used in nuclear transfer, and have confirmed the authenticity of Dolly.

Date: 1998
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DOI: 10.1038/28524

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