Role of citron kinase as a target of the small GTPase Rho in cytokinesis

Pascal Madaule, Masatoshi Eda, Naoki Watanabe, Kazuko Fujisawa, Toshiyuki Matsuoka, Haruhiko Bito, Toshimasa Ishizaki and Shuh Narumiya ()
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Pascal Madaule: Kyoto University Faculty of Medicine
Masatoshi Eda: Kyoto University Faculty of Medicine
Naoki Watanabe: Kyoto University Faculty of Medicine
Kazuko Fujisawa: Kyoto University Faculty of Medicine
Toshiyuki Matsuoka: Kyoto University Faculty of Medicine
Haruhiko Bito: Kyoto University Faculty of Medicine
Toshimasa Ishizaki: Kyoto University Faculty of Medicine
Shuh Narumiya: Kyoto University Faculty of Medicine

Nature, 1998, vol. 394, issue 6692, 491-494

Abstract: Abstract During mitosis, a ring containing actin and myosin appears beneath the equatorial surface of animal cells. This ring then contracts, forms a cleavage furrow and divides the cell1,2,3, a step known as cytokinesis. The two daughter cells often remain connected by an intercellular bridge which contains a refringent structure known as the midbody4,5. How the appearance of this ring is regulated is unclear, although the small GTPase Rho, which controls the formation of actin structures6,7, is known to be essential8,9,10. Protein kinases are also thought to participate in cytokinesis1,2,3,11,12. We now show that a splice variant of a Rho target protein, named citron13, contains a protein kinase domain that is related to the Rho-associated kinases ROCK14 and ROK15,16,17, which regulate myosin-based contractility18,19,20,21. Citron kinase localizes to the cleavage furrow and midbody of HeLa cells; Rho is also localized in the midbody. We find that overexpression of citron mutants results in the production of multinucleate cells and that a kinase-active mutant causes abnormal contraction during cytokinesis. We propose that citron kinase regulates cytokinesis at a step after Rho in the contractile process.

Date: 1998
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DOI: 10.1038/28873

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