Structure of the metal-ion-activated diphtheria toxin repressor/ tox operator complex

André White, Xiaochun Ding, Johanna C. vanderSpek, John R. Murphy and Dagmar Ringe ()
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André White: Rosenstiel Basic Medical Sciences Research Center MS029
Xiaochun Ding: Rosenstiel Basic Medical Sciences Research Center MS029
Johanna C. vanderSpek: Boston University School of Medicine
John R. Murphy: Boston University School of Medicine
Dagmar Ringe: Rosenstiel Basic Medical Sciences Research Center MS029

Nature, 1998, vol. 394, issue 6692, 502-506

Abstract: Abstract The virulent phenotype of the pathogenic bacterium Corynebacterium diphtheriae is conferred by diphtheria toxin, whose expression is an adaptive response to low concentrations of iron. The expression of the toxin gene (tox) is regulated by the repressor DtxR (ref. 1), which is activated by transition metal ions. X-ray crystal structures of DtxR with2,3,4,5 and without (apo-form2) its coordinated transition metal ion have established the general architecture of the repressor, identified the location of the metal-binding sites, and revealed a metal-ion-triggered subunit–subunit ‘caliper-like’ conformational change. Here we report thethree-dimensional crystal structure of the complex between a biologically active Ni(ii)-bound DtxR(C102D) mutant, in whicha cysteine is replaced by an aspartate at residue 102, and a 33-base-pair DNA segment containing the toxin operator tox O. This structure shows that DNA interacts with two dimeric repressor proteins bound to opposite sides of the tox operator. We propose that a metal-ion-induced helix-to-coil structural transition in the amino-terminal region of the protein is partly responsible for the unique mode of repressor activation by transition metal ions.

Date: 1998
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DOI: 10.1038/28893

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