Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling

Karuna Sampath, Amy L. Rubinstein, Abby M. S. Cheng, Jennifer O. Liang, Kimberly Fekany, Lilianna Solnica-Krezel, Vladimir Korzh, Marnie E. Halpern and Christopher V. E. Wright
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Karuna Sampath: Vanderbilt University School of Medicine
Amy L. Rubinstein: Carnegie Institution of Washington
Abby M. S. Cheng: Vanderbilt University School of Medicine
Jennifer O. Liang: Carnegie Institution of Washington
Kimberly Fekany: Vanderbilt University
Lilianna Solnica-Krezel: Vanderbilt University
Vladimir Korzh: Institute of Molecular Agrobiology, National University of Singapore
Marnie E. Halpern: Carnegie Institution of Washington
Christopher V. E. Wright: Vanderbilt University School of Medicine

Nature, 1998, vol. 395, issue 6698, 185-189

Abstract: Abstract Zebrafish cyclops (cyc) mutations cause deficiencies in the dorsal mesendoderm1,2 and ventral neural tube3,4, leading to neural defects and cyclopia5,6. Here we report that cyc encodes a transforming growth factor-β (TGF-β)-related intercellular signalling molecule that is similar to mouse nodal7. cyc is expressed in dorsal mesendoderm at gastrulation and in the prechordal plate until early somitogenesis. Expression reappears transiently in the left lateral-plate mesoderm, and in an unprecedented asymmetric pattern in the left forebrain. Injection of cyc RNA non-autonomously restores sonic hedgehog -expressing cells of the ventral brain and floorplate that are absent in cyc mutants, whereas inducing activities are abolished by cycm294, a mutation of a conserved cysteine in the mature ligand. Our results indicate that cyc provides an essential non-cell-autonomous signal at gastrulation, leading to induction of the floorplate and ventral brain.

Date: 1998
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DOI: 10.1038/26020

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