Crystal structure of a hepatitis delta virus ribozyme

Adrian R. Ferré-D'Amaré, Kaihong Zhou and Jennifer A. Doudna ()
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Adrian R. Ferré-D'Amaré: Yale University
Kaihong Zhou: Howard Hughes Medical Institute, Yale University
Jennifer A. Doudna: Yale University

Nature, 1998, vol. 395, issue 6702, 567-574

Abstract: Abstract The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 Å resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5′-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.

Date: 1998
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DOI: 10.1038/26912

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