A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication

Corrado Santocanale and John F. X. Diffley ()
Additional contact information
Corrado Santocanale: Imperial Cancer Research Fund, Clare Hall Laboratories
John F. X. Diffley: Imperial Cancer Research Fund, Clare Hall Laboratories

Nature, 1998, vol. 395, issue 6702, 615-618

Abstract: Abstract DNA replication in eukaryotic cells initiates from many replication origins1 which fire throughout the S phase of the cell cycle in a predictable pattern: some origins fire early, others late2. Little is known about how the initiation of DNA replication and the elongation of newly synthesized DNA strands are coordinated during S phase. Here we show that, in budding yeast, hydroxyurea, which blocks the progression of replication forks from early-firing origins, also inhibits the firing of late origins. These late origins are maintained in the initiation-competent prereplicative state for extended periods. The block to late origin firing is an active process and is defective in yeast with mutations in the rad53 and mec1 checkpoint genes, indicating that regulation of late origin firing may also be an important component of the ‘intra-S-phase’ checkpoint3 and may aid cell survival under adverse conditions.

Date: 1998
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/27001 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:395:y:1998:i:6702:d:10.1038_27001

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/27001

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().