Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex

Jeffrey K. Tong, Christian A. Hassig, Gavin R. Schnitzler, Robert E. Kingston and Stuart L. Schreiber ()
Additional contact information
Jeffrey K. Tong: Howard Hughes Medical Institute, Molecular and Cellular Biology, Harvard University
Christian A. Hassig: Howard Hughes Medical Institute, Molecular and Cellular Biology, Harvard University
Gavin R. Schnitzler: Massachusetts General Hospital
Robert E. Kingston: Massachusetts General Hospital
Stuart L. Schreiber: Howard Hughes Medical Institute, Molecular and Cellular Biology, Harvard University

Nature, 1998, vol. 395, issue 6705, 917-921

Abstract: Abstract The dynamic assembly and remodelling of eukaryotic chromosomes facilitate fundamental cellular processes such as DNA replication and gene transcription. The repeating unit of eukaryotic chromosomes is the nucleosome core, consisting of DNA wound about a defined octamer of histone proteins1. Two enzymatic processes that regulate transcription by targeting elements of the nucleosome include ATP-dependent nucleosome remodelling and reversible histone acetylation2,3. The histone deacetylases, however, are unable to deacetylate oligonucleosomal histones in vitro4. The protein complexes that mediate ATP-dependent nucleosome remodelling and histone acetylation/deacetylation in the regulation of transcription were considered to be different, although it has recently been suggested that these activities might be coupled5. We report here the identification and functional characterization of a novel ATP-dependent nucleosome remodelling activity that is part of an endogenous human histone deacetylase complex. This activity is derived from the CHD3 and CHD4 proteins which contain helicase/ATPase domains found in SWI2-related chromatin remodelling factors, and facilitates the deacetylation of oligonucleosomal histones in vitro. We refer to this complex as the nucleosome remodelling and deacetylating (NRD) complex. Our results establish a physical and functional link between the distinct chromatin-modifying activities of histone deacetylases and nucleosome remodelling proteins.

Date: 1998
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/27699 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:395:y:1998:i:6705:d:10.1038_27699

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/27699

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().