Interdomain communication regulating ligand binding by PPAR-γ

Dalei Shao, Shamina M. Rangwala, Shannon T. Bailey, Samuel L. Krakow, Mauricio J. Reginato and Mitchell A. Lazar ()
Additional contact information
Dalei Shao: Diabetes, and Metabolism
Shamina M. Rangwala: Diabetes, and Metabolism
Shannon T. Bailey: Diabetes, and Metabolism
Samuel L. Krakow: Diabetes, and Metabolism
Mauricio J. Reginato: Departments of Pharmacology
Mitchell A. Lazar: Diabetes, and Metabolism

Nature, 1998, vol. 396, issue 6709, 377-380

Abstract: Abstract Binding to receptors in the cell nucleus is crucial for the action of lipophilic hormones and ligands. PPAR-γ (for peroxisome proliferator-activated receptor) is a nuclear hormone receptor that mediates adipocyte differentiation1,2 and modulates insulin sensitivity3, cell proliferation4 and inflammatory processes5,6. PPAR-γ ligands have been implicated in the development of atherogenic foam cells7 and as potential cancer treatments8. Transcriptional activity of PPAR-γ is induced by binding diverse ligands, including natural fatty acid derivatives9,10,11, antidiabetic thiazolidinediones12, and non-steroidal anti-inflammatory drugs13. Ligand binding by PPAR-γ, as well as by the entire nuclear-receptor superfamily, is an independent property of the carboxy-terminal ligand-binding domain (LBD) of the receptor14,15. Here we show that ligand binding by PPAR-γ is regulated by intramolecular communication between its amino-terminal A/B domain and its carboxy-terminal LBD. Modification of the A/B domain, for example by physiological phosphorylation by MAP kinase, reduces ligand-binding affinity, thus negatively regulating the transcriptional and biological functions of PPAR-γ. The ability of the A/B domain to regulate ligand binding has important implications for the evaluation and mechanism of action of potentially therapeutic ligands that bind PPAR-γ and that are likely to extend to other members of the nuclear-receptor superfamily.

Date: 1998
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DOI: 10.1038/24634

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