Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps

Yoshikazu Suzuki, Takuo Yasunaga, Reiko Ohkura, Takeyuki Wakabayashi and Kazuo Sutoh ()
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Yoshikazu Suzuki: School of Arts and Sciences, University of Tokyo
Takuo Yasunaga: School of Science, University of Tokyo
Reiko Ohkura: School of Arts and Sciences, University of Tokyo
Takeyuki Wakabayashi: School of Science, University of Tokyo
Kazuo Sutoh: School of Arts and Sciences, University of Tokyo

Nature, 1998, vol. 396, issue 6709, 380-383

Abstract: Abstract In muscle, the myosin head (‘crossbridge’) performs the ‘working stroke’, in which ATP is hydrolysed to generate the sliding of actin and myosin filaments. The myosin head consists of a globular motor domain and a long lever-arm domain. The ‘lever-arm hypothesis’1,2,3,4,5 predicts that during the working stroke, the lever-arm domain tilts against the motor domain, which is bound to actin in a fixed orientation. To detect this working stroke in operation, we constructed fusion proteins by connecting Aequorea victoria green fluorescent protein and blue fluorescent protein6,7,8 to the amino and carboxyl termini of the motor domain of myosin II of Dictyostelium discoideum, a soil amoeba, and measured the fluorescence resonance energy transfer between the two fluorescent proteins. We show here that the carboxy-terminal fluorophore swings at the isomerization step of the ATP hydrolysis cycle, and then swings back at the subsequent step in which inorganic phosphate is released, thereby mimicking the swing of the lever arm. The swing at the phosphate-release step may correspond to the working stroke, and the swing at the isomerization step to the recovery stroke.

Date: 1998
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DOI: 10.1038/24640

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