Functional interaction between InsP3 receptors and store-operated Htrp3 channels

Kirill Kiselyov, Xin Xu, Galina Mozhayeva, Tuan Kuo, Isaac Pessah, Gregory Mignery, Xi Zhu, Lutz Birnbaumer# and Shmuel Muallem ()
Additional contact information
Kirill Kiselyov: University of Texas Southwestern Medical Center at Dallas
Xin Xu: University of Texas Southwestern Medical Center at Dallas
Galina Mozhayeva: Institute of Cytology
Tuan Kuo: Wayne State University
Isaac Pessah: School of Veterinary Medicine, University of California
Gregory Mignery: Stritch School of Medicine, Loyola University Chicago
Xi Zhu: The Ohio State University
Lutz Birnbaumer#: Cell and Developmental Biology, University of California
Shmuel Muallem: University of Texas Southwestern Medical Center at Dallas

Nature, 1998, vol. 396, issue 6710, 478-482

Abstract: Abstract Calcium ions are released from intracellular stores in response to agonist-stimulated production of inositol 1,4,5-trisphosphate (InsP3), a second messenger generated at the cell membrane. Depletion of Ca2+ from internal stores triggers a capacitative influx of extracellular Ca2+ across the plasma membrane1,2. The influx of Ca2+ can be recorded as store-operated channels (SOC) in the plasma membrane or as a current known as the Ca2+-release-activated current (Icrac)3,4,5. A critical question in cell signalling is how SOC and Icrac sense and respond to Ca2+-store depletion: in one model, a messenger molecule is generated that activates Ca2+ entry in response to store depletion1,6; in an alternative model7, InsP3 receptors in the stores are coupled to SOC and Icrac. The mammalian Htrp3 protein8 forms a well defined store-operated channel8,9 and so provides a suitable system for studying the effect of Ca2+-store depletion on SOC and Icrac. We show here that Htrp3 channels stably expressed in HEK293 cells are in a tight functional interaction with the InsP3 receptors. Htrp3 channels present in the same plasma membrane patch can be activated by Ca2+ mobilization in intact cells and by InsP3 in excised patches. This activation of Htrp3 by InsP3 is lost on extensive washing of excised patches but is restored by addition of native or recombinant InsP3-bound InsP3 receptors. Our results provide evidence for the coupling hypothesis7, in which InsP3 receptors activated by InsP3 interact with SOC and regulate Icrac.

Date: 1998
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DOI: 10.1038/24890

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